TB-500 Dosage Calculator: Calculate Your Exact Dose 2026

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Figuring out the right TB-500 dosage can feel overwhelming. Between vial sizes, reconstitution volumes, and loading versus maintenance phases, there are a lot of variables to get right.

Based on our research and testing multiple peptide calculators, the standard TB-500 dosage is 2-5mg injected twice weekly during the loading phase (4-6 weeks), then 2-5mg once weekly for maintenance.

I have spent considerable time comparing dosing protocols from researchers and forums to create this comprehensive TB-500 dosage calculator guide. Below, you will find everything you need to calculate your exact dose, reconstitute your peptide correctly, and understand the differences between loading and maintenance protocols.

Important: TB-500 (Thymosin Beta-4) is not FDA-approved for human use. This content is for research and educational purposes only.

TB-500 Dosage Calculator

Use this calculator to determine your exact TB-500 dose based on your vial size, reconstitution volume, and desired dosage. The calculator shows you exactly how many units to draw in your syringe.

Step 1: Enter Your Vial and Reconstitution Details

Input Field	Common Values	Your Value
Vial Size (mg)	5mg or 10mg	_____ mg
Bacteriostatic Water Added (ml)	1ml or 2ml	_____ ml
Desired Dose (mg)	2mg, 2.5mg, 5mg	_____ mg
Syringe Type	100 unit insulin syringe	_____ units

Step 2: Calculate Your Concentration

The formula is straightforward: divide your vial size by the water volume to get your concentration.

Concentration Formula: Vial Size (mg) / Water Added (ml) = Concentration (mg/ml)

Vial Size	Water Added	Concentration
5mg	1ml	5mg/ml
5mg	2ml	2.5mg/ml
10mg	2ml	5mg/ml
10mg	4ml	2.5mg/ml

Step 3: Calculate Units to Inject

Once you know your concentration, divide your desired dose by the concentration, then multiply by 100 to get syringe units.

Units Formula: (Desired Dose / Concentration) x 100 = Syringe Units

Desired Dose	Concentration	ML to Inject	Syringe Units (100u)
2mg	2.5mg/ml	0.80ml	80 units
2.5mg	2.5mg/ml	1.00ml	100 units
2mg	5mg/ml	0.40ml	40 units
2.5mg	5mg/ml	0.50ml	50 units
5mg	5mg/ml	1.00ml	100 units

Quick Reference: How Long Does a Vial Last?

Knowing how long your vial will last helps with budgeting and protocol planning.

Vial Size	Protocol	Dose Per Injection	Injections Per Vial	Duration
5mg	Loading (2x/week)	2.5mg	2	1 week
5mg	Maintenance (1x/week)	2.5mg	2	2 weeks
10mg	Loading (2x/week)	2.5mg	4	2 weeks
10mg	Maintenance (1x/week)	2.5mg	4	4 weeks

How to Reconstitute TB-500?

Reconstitution is the process of mixing the lyophilized (freeze-dried) TB-500 powder with bacteriostatic water to create an injectable solution. Proper reconstitution is essential for accurate dosing.

What You Need?

• TB-500 vial (5mg or 10mg lyophilized powder)
• Bacteriostatic water (BAC water with 0.9% benzyl alcohol)
• Insulin syringes (100 unit, 29-31 gauge)
• Alcohol swabs for sterilization

Step-by-Step Reconstitution Process

1. Clean your workspace: Wipe down your work area and wash your hands thoroughly. Use a clean, well-lit surface.
2. Sterilize the vial tops: Use an alcohol swab to clean the rubber stopper on both the TB-500 vial and bacteriostatic water vial. Let them air dry for 30 seconds.
3. Draw the bacteriostatic water: Using an insulin syringe, draw your desired amount of BAC water. For a 5mg vial, 2ml is recommended for easy dosing calculations.
4. Inject water slowly: Insert the needle into the TB-500 vial at an angle, aiming the stream at the glass wall rather than directly onto the powder. Add water slowly over 30-60 seconds.
5. Let it dissolve: Do not shake the vial. Gently swirl if needed, but the powder should dissolve on its own within 5-10 minutes. The solution should be clear.
6. Label and store: Write the date of reconstitution and concentration on the vial. Store in the refrigerator at 36-46F (2-8C).

Reconstitution Quick Reference Table

Vial Size	Recommended Water	Resulting Concentration	Shelf Life (Refrigerated)
5mg	2ml	2.5mg/ml (2500mcg/ml)	Up to 30 days
10mg	2ml	5mg/ml (5000mcg/ml)	Up to 30 days
10mg	4ml	2.5mg/ml (2500mcg/ml)	Up to 30 days

Pro Tip: Using 2ml of water for a 5mg vial gives you a 2.5mg/ml concentration. This makes calculations simple: each 0.1ml (10 units) equals 250mcg, and each 1ml (100 units) equals 2.5mg.

Common Reconstitution Mistakes to Avoid

• Spraying water directly on powder: This can damage the peptide structure. Always aim at the vial wall.
• Shaking the vial: Aggressive shaking can denature the peptide. Gentle swirling is acceptable.
• Using sterile water instead of bacteriostatic water: BAC water contains a preservative that extends shelf life. Sterile water must be used within 24 hours.
• Storing at room temperature: Once reconstituted, TB-500 degrades quickly at room temperature. Always refrigerate.

TB-500 Dosing Protocols

TB-500 dosing is typically divided into two phases: a loading phase where higher doses saturate the system, followed by a maintenance phase with lower doses. Research protocols suggest 2-5mg twice weekly during loading, transitioning to 2-5mg once weekly for maintenance.

Loading Phase Protocol

The loading phase involves higher, more frequent doses to build up TB-500 levels in the body. This phase typically lasts 4-6 weeks.

Parameter	Recommended Range	Notes
Duration	4-6 weeks	Some protocols extend to 8 weeks
Frequency	Twice weekly	Every 3-4 days (e.g., Monday/Thursday)
Dose Per Injection	2-5mg	2.5mg is most common
Weekly Total	4-10mg	5mg/week (2x 2.5mg) is typical

During the loading phase, users report that staying consistent with injection timing may help with results. Missing doses or irregular scheduling appears to reduce effectiveness based on forum feedback.

Maintenance Phase Protocol

After completing the loading phase, the maintenance phase uses lower doses to sustain effects. This phase can continue indefinitely or be cycled.

Parameter	Recommended Range	Notes
Duration	8-12+ weeks	Some run ongoing maintenance
Frequency	Once weekly	Same day each week for consistency
Dose Per Injection	2-5mg	2.5mg is common for maintenance
Weekly Total	2-5mg	Half of loading phase dosage

Loading vs Maintenance Comparison

Phase	Duration	Frequency	Dose/Injection	Weekly Total
Loading	4-6 weeks	2x per week	2-5mg	4-10mg
Maintenance	8-12+ weeks	1x per week	2-5mg	2-5mg

Weight-Based Dosing Considerations

While most TB-500 protocols use fixed doses regardless of body weight, some researchers adjust dosing based on weight. Here are general guidelines based on forum discussions and research protocols:

Body Weight	Loading Dose (2x/week)	Maintenance Dose (1x/week)
Under 150 lbs (68 kg)	2-2.5mg	2mg
150-200 lbs (68-91 kg)	2.5-3mg	2-2.5mg
200-250 lbs (91-113 kg)	3-4mg	2.5-3mg
Over 250 lbs (113+ kg)	4-5mg	3-5mg

These are general guidelines based on user reports. Individual responses may vary, and starting with lower doses to assess tolerance is a common approach.

Syringe Unit Conversion Table

Converting milliliters to syringe units is essential for accurate dosing. Standard insulin syringes hold 1ml (100 units), with each unit marking representing 0.01ml.

Understanding Syringe Units

An insulin syringe marked in units makes dosing easier once you understand the conversion:

• 100 units = 1ml
• 50 units = 0.5ml
• 10 units = 0.1ml
• 1 unit = 0.01ml

Complete Conversion Table (2.5mg/ml Concentration)

Using the most common concentration of 2.5mg/ml (5mg vial + 2ml water):

Desired Dose	ML to Draw	Syringe Units
0.5mg (500mcg)	0.20ml	20 units
1mg (1000mcg)	0.40ml	40 units
1.5mg (1500mcg)	0.60ml	60 units
2mg (2000mcg)	0.80ml	80 units
2.5mg (2500mcg)	1.00ml	100 units

Complete Conversion Table (5mg/ml Concentration)

For a higher concentration of 5mg/ml (10mg vial + 2ml water):

Desired Dose	ML to Draw	Syringe Units
0.5mg (500mcg)	0.10ml	10 units
1mg (1000mcg)	0.20ml	20 units
2mg (2000mcg)	0.40ml	40 units
2.5mg (2500mcg)	0.50ml	50 units
5mg (5000mcg)	1.00ml	100 units

Which Syringe Size to Use?

For most TB-500 doses, a standard 1ml (100 unit) insulin syringe works well. For doses of 30 units or less, a 0.3ml (30 unit) syringe provides more precision.

Syringe Size	Best For	Needle Gauge
0.3ml (30 unit)	Small doses under 30 units	29-31G
0.5ml (50 unit)	Medium doses 30-50 units	29-31G
1ml (100 unit)	Standard TB-500 doses	29-31G

How to Administer TB-500?

TB-500 is typically administered via subcutaneous injection, though intramuscular injection is also used. Subcutaneous injections are simpler and preferred by most researchers.

Subcutaneous Injection Method

Subcutaneous means injecting into the fatty tissue just below the skin. This is the most common administration route for TB-500.

1. Choose your injection site: Common areas include the abdomen (2 inches from navel), upper thigh, or back of the arm. Rotate sites to prevent irritation.
2. Clean the area: Wipe the injection site with an alcohol swab and let it air dry.
3. Pinch the skin: Use your non-dominant hand to pinch about an inch of skin and fat.
4. Insert the needle: At a 45-90 degree angle, insert the needle fully into the pinched skin.
5. Inject slowly: Push the plunger steadily and completely. Injecting too fast can cause discomfort.
6. Withdraw and dispose: Remove the needle and apply light pressure if needed. Dispose of the syringe properly.

Intramuscular Injection Option

Some researchers prefer intramuscular (IM) injection, particularly when targeting a specific injury area. However, this method requires longer needles and proper technique.

Common IM injection sites include:

• Deltoid (shoulder)
• Vastus lateralis (outer thigh)
• Gluteus (upper outer quadrant)

Injection Site Rotation

Rotating injection sites helps prevent tissue irritation and allows proper healing. A simple rotation pattern:

• Day 1: Left side of abdomen
• Day 2: Right side of abdomen
• Day 3: Left thigh
• Day 4: Right thigh

TB-500 and BPC-157 Combination Protocol

Combining TB-500 with BPC-157 is one of the most popular peptide stacking protocols. Research suggests these peptides may work synergistically, with TB-500 supporting tissue regeneration and BPC-157 potentially enhancing healing at the cellular level.

Why Combine TB-500 and BPC-157?

Based on forum discussions and anecdotal reports, researchers combine these peptides because:

• Different mechanisms: TB-500 works primarily through actin binding and cell migration, while BPC-157 may affect growth hormone receptors and blood vessel formation through different pathways.
• Complementary timing: BPC-157 is often used daily for acute issues, while TB-500 is used less frequently for systemic effects.
• Reported synergy: Users frequently report enhanced results when stacking compared to using either peptide alone.

Combination Dosing Protocol

Peptide	Loading Phase	Maintenance Phase	Frequency
TB-500	2.5mg	2.5mg	2x/week (loading), 1x/week (maintenance)
BPC-157	250-500mcg	250mcg	Daily or twice daily

Sample 8-Week Stack Protocol

Weeks 1-4 (Loading Phase):

• TB-500: 2.5mg twice weekly (Monday and Thursday)
• BPC-157: 250mcg twice daily (morning and evening)

Weeks 5-8 (Maintenance Phase):

• TB-500: 2.5mg once weekly (Monday)
• BPC-157: 250mcg once daily (morning)

Important Considerations

• Separate injections: While some users mix peptides in the same syringe, most researchers recommend separate injections to ensure accurate dosing.
• Different storage: BPC-157 and TB-500 have similar storage requirements (refrigerated after reconstitution), but keep them in separate vials.
• Injection timing: There is no evidence that injection timing relative to each other matters significantly.

Where to Buy TB-500?

Quality matters significantly when purchasing TB-500 for research purposes. Based on our research, we have identified five reputable suppliers that offer third-party tested TB-500 with certificates of analysis.

All providers listed below sell TB-500 for research purposes only and provide purity verification.

1. Limitless Biotech – Best Value

Limitless Biotech stands out for offering the most competitive pricing starting at $54.99 for a 5mg vial. They manufacture in Pensacola, Florida and provide extensive documentation including COA, HPLC, and mass spectrometry verification.

Their bulk discount structure is among the best: 5% off for 5-9 vials, 10% for 10-14 vials, and 15% for 15+ vials. Same-day shipping is available for orders placed early.

2. Core Peptides – Best for Bulk Orders

Core Peptides offers TB-500 with greater than 99% purity verified by HPLC and mass spectrometry. Their 5mg vials are priced at $78.00, with 10mg vials available for $140.00.

The free shipping threshold of $200 makes them ideal for larger research orders. Their bulk discounts of 5% for 5-8 vials and 10% for 9+ vials add additional value.

3. Peptide Sciences – Best Documentation

Peptide Sciences is known for their thorough scientific approach, providing extensive documentation including full certificates of analysis with HPLC and mass spectrometry data.

Based in Boca Raton, Florida, they offer bulk pricing: 6% off for 3+ vials, 10% off for 5+ vials, and 12% off for 10+ vials. Their rewards program provides additional value for repeat researchers.

4. Biotech Peptides – USA Made

Biotech Peptides offers USA-synthesized TB-500 with 99% purity verification through HPLC and mass spectrometry. They provide free shipping on orders exceeding $200.

While their product pages are less detailed than some competitors, they maintain research-grade quality standards with verified purity.

5. Behemoth Labz – Highest Purity

Behemoth Labz claims the highest purity at 99.99%, with every batch verified by third-party testing. They offer competitive pricing with fast shipping from their USA facility.

While a newer entrant compared to some established competitors, their commitment to batch testing and purity verification has earned them a growing reputation.

What to Look for When Buying TB-500?

• Third-party testing: Look for COA (Certificate of Analysis), HPLC verification, and mass spectrometry data
• Purity level: Minimum 99% purity is standard for research-grade peptides
• USA manufacturing: Domestic production often means better quality control
• Proper storage during shipping: Cold chain shipping helps maintain peptide integrity
• Clear labeling: Research use only designation is required for legal sale

Frequently Asked Questions

Final Thoughts

Accurate TB-500 dosing requires understanding your vial concentration, proper reconstitution technique, and the difference between loading and maintenance phases. Use the calculator and reference tables in this guide to determine your exact syringe units for each injection.

The key takeaways for TB-500 dosing are:

• Standard loading dose: 2-5mg twice weekly for 4-6 weeks
• Standard maintenance dose: 2-5mg once weekly
• Most common concentration: 2.5mg/ml (5mg vial + 2ml BAC water)
• Store reconstituted TB-500 refrigerated for up to 30 days
• Quality matters: choose providers with third-party testing

When sourcing TB-500, prioritize suppliers that provide certificates of analysis, HPLC verification, and clear purity documentation. The providers listed in this guide all meet these quality standards for research purposes.

Disclaimer: TB-500 (Thymosin Beta-4) is not FDA-approved for human use. This article is for informational and research purposes only and does not constitute medical advice. Always consult a licensed healthcare provider before using any peptides or research compounds.